STIMULATING HAIR GROWTH BY HELIUM-NEON LASER IN RATS
Abstract
We performed a study to determine whether 632.8 nm helium-neon (He-Ne) laser radiation would affect hair growth of the skin after the hairs had been pull out in 18 female rats, 8 weeks of aged. The four skin areas, 2 cm in diameter each, the hairs were pull out at the back of all rats after ketamine hydrochloride anesthesia. To determine the rate of hair growth and optimum daily energy density irradiance, energy density of 1.35, 2.70 and 4.05 J/cm2/day of He-Ne laser were delivered for two weeks to area I (15 mins every 24 hrs), area II, two repeated exposures at 12 hrs interval and area III, three repeated exposures at 8 hrs interval, respectively. The control site (area IV) was not irradiated but received placebo light. Additional to the observation of the reaction at all areas where the hairs were pull out, length of hairs on them were measured daily by capillary tube and magnifying lens. The results shown that normal rate of hair growth was 0.27 mm/day while the growth rate at the irradiated areas with 1.35, 2.70 and 4.05 J/cm2/day were 0.42, 0.62 and 0.36 mm/day, respectively. 2 repeated daily exposures were the optimum energy density for stimulation of hair growth. Hair growth in the control site was the same rate as 1 exposure site and 3 repeated exposures site. Hair follicles needed some energy density of He-Ne laser radiation to initiate hair growth in rats.
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