COMPARISON OF SERUM THYROGLOBULIN MEASUREMENTS BY RADIOIMMUNOASSAY AND IMMUNORADIOMETRIC ASSAY
Abstract
Serum thyroglobulin (Tg) concentrations are widely used as a tumour marker for monitoring the patients with differentiated thyroid carcinoma. Precise and reproducible Tg measurements are critical, especially when patients are judged to have a high risk for recurrence. The clinical utility of two different Tg methods (RIA and IRMA) was compared and evaluated focusing on measurement methodology. Therefore, complete quality control profiles were performed and assessed. The results revealed that the sensitivity (low detection limit) of the assays for Tg-RIA (DPC) and Tg-IRMA (CIS) was 2.28 ng/ml and 0.62 ng/ml, respectively. The assay precision of both intra-and inter-assays had coefficient of variation (C. V.) of 5.49-15.63% for Tg-RIA and 5.56-8.27% for Tg-IRMA. The accuracy of the two assays was 96.9-121.3% for Tg-RIA and 97.6- 104.3% for Tg-IRMA. No cross-reaction and no drift effect were obtained in both assays. The results showed complete parallelism between Tg standards and serial dilutions of Tg-containing serum. The hook effects were indicated with both assays at very high concentration of Tg. The quality of Tg-IRMA (CIS) was proved to be superior to MAY. - AUG. 2000. Volume VI Number II Tg-RIA (DPC).
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